Whole-cell and perforated patch-clamp methods to investigate ion channel function.

Ion channel currents

Cell-attached and excised-patch capacitance measurements to study single exocytotic and endocytotic vesicles

Ultra-high resolution capacitance

Whole-islet and single cell imaging for localization of proteins or for functional calcium and membrane imaging studies (i.e. TIRF microscopy).

Imaging

Standard molecular biology such as RT-PCR, molecular cloning, northern blot, western blot, siRNA, and recombinant adenovirus

Molecular biology

A range of cell and animal models are used, including insulinoma cells (INS-1) and isolated rodent islets. Also, transgenic animals and animal models of type 2 diabetes are used.

Cells and animals

BACKGROUND

PROJECTS

TECHNIQUES

THE LAB

THE PEOPLE

The MacDonald Islet Biology Lab

bcell.org

bcell.org

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