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Pancreatic islets are composed of numerous cell types, including insulin secreting b-cells and glucagon secreting a-cells. Since the glucagon secreting pancreatic a-cells are presently under-studied, and may contribute significantly to diabetes, we are initiating projects to examine the regulation of a-cells cell function. Using both electrophysiological and imaging techniques.
See the recent paper here. |
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Pancreatic b-cells release insulin through the regulated exocytosis of large dense-core vesicles (LDCV). b-cells also secrete neurotransmitters with important autocrine and paracrine effects by the regulated exocytosis of synaptic-like microvesicles (SLMVs). We are therefore investigating the exocytosis and recycling of LDCVs and SLMVs at the level of single vesicles using high resolution capacitance measurements. See the recent papers on exocytosis, endocytosis and fusion pores. |
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We are establishing a research program to focus on the regulation of Kv channel biophysical properties. This will build on extensive previous work that examined the role of these channels in insulin secretion. We will initially examine the regulation of these channels by metabolic and physical interactions. |
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p110g is the only known PI3 kinase isoform that is directly activated by G-protein coupled receptors. This study examines the role of p110g in insulin secretion using transgenic p110g -/- mice and acute knockdown with siRNAs. Current work is funded by the CDA and is being performed in a collaboration with Dr. P Brubaker’s group in Toronto.
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See a list of published reviews and collaborative projects. |
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We are presently embarking on a new line of research, examining methods to improve islet graft function for transplantation in type 1 diabetes. In collaboration with Dr. Jamie Joseph at the University of Waterloo, we are examining novel strategies for gene delivery prior to or during islet isolation. Gene’s of interest include those to enhance islet graft function and survival. |
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BACKGROUND |
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PROJECTS |
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TECHNIQUES |
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THE LAB |
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THE PEOPLE |
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The MacDonald Islet Biology Lab |
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bcell.org |
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bcell.org |
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